[HTML][HTML] Molecular interactions between two global regulators, sar and agr, in Staphylococcus aureus
Y Chien, AL Cheung - Journal of Biological Chemistry, 1998 - ASBMB
The expression of many virulence determinants inStaphylococcus aureus is controlled by
regulatory loci such as agr and sar. We have previously shown that the SarA protein is
required for optimal transcription of RNAII and RNAIII in the agr locus. To define the specific
molecular interaction, we overexpressed SarA as a glutathioneS-transferase (GST) fusion
protein by cloning the 372-base pair (bp) sarA gene into the vector. The purified GST-SarA
as well as cleaved SarA were able to bind specifically to the P2, P3, and the combined P2 …
regulatory loci such as agr and sar. We have previously shown that the SarA protein is
required for optimal transcription of RNAII and RNAIII in the agr locus. To define the specific
molecular interaction, we overexpressed SarA as a glutathioneS-transferase (GST) fusion
protein by cloning the 372-base pair (bp) sarA gene into the vector. The purified GST-SarA
as well as cleaved SarA were able to bind specifically to the P2, P3, and the combined P2 …