[HTML][HTML] Deficiency in Msh2 affects the efficiency and local sequence specificity of immunoglobulin class-switch recombination: parallels with somatic hypermutation

MR Ehrenstein, MS Neuberger - The EMBO journal, 1999 - embopress.org
MR Ehrenstein, MS Neuberger
The EMBO journal, 1999embopress.org
Results Immunoglobulin heavy chain class switching can occur in Msh2-deficient mice:
there is little difference between Msh2+/− and Msh2−/− siblings in the abundance of the
various serum isotypes (Figure 1A). However, our previous work revealed a consistent 3-to 4-
fold reduction in the titre of NP-specific IgG1 (but not IgM) antibody in NP–chicken γ-globulin
(NP–CG)-immunized Msh2-deficient mice at day 8 and day 12 as compared with controls
(Rada et al., 1998).
Results
Immunoglobulin heavy chain class switching can occur in Msh2-deficient mice: there is little difference between Msh2+/− and Msh2−/− siblings in the abundance of the various serum isotypes (Figure 1A). However, our previous work revealed a consistent 3-to 4-fold reduction in the titre of NP-specific IgG1 (but not IgM) antibody in NP–chicken γ-globulin (NP–CG)-immunized Msh2-deficient mice at day 8 and day 12 as compared with controls (Rada et al., 1998).
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