[HTML][HTML] Dissociation between stem cell phenotype and NOD/SCID repopulating activity in human peripheral blood CD34+ cells after ex vivo expansion
GH Danet, HW Lee, JL Luongo, MC Simon… - Experimental …, 2001 - Elsevier
OBJECTIVES: The relationship between phenotype and function in ex vivo–cultured human
hematopoietic stem cells (HSC) remains poorly understood. We investigated the effects of a
short-term serum-free culture on the relationship between stem cell phenotype, cell division
history, and function in human CD34+ cells. METHODS: G-CSF–mobilized peripheral
CD34+ cells were cultured for 4 days with stem cell factor, flt-3 ligand, and thrombopoietin.
The phenotype (CD34, CD38, HLA-DR, c-kit), cell division history, colony-forming cell (CFC) …
hematopoietic stem cells (HSC) remains poorly understood. We investigated the effects of a
short-term serum-free culture on the relationship between stem cell phenotype, cell division
history, and function in human CD34+ cells. METHODS: G-CSF–mobilized peripheral
CD34+ cells were cultured for 4 days with stem cell factor, flt-3 ligand, and thrombopoietin.
The phenotype (CD34, CD38, HLA-DR, c-kit), cell division history, colony-forming cell (CFC) …