[HTML][HTML] Bcl-2 and Bcl-xL inhibit CD95-mediated apoptosis by preventing mitochondrial release of Smac/DIABLO and subsequent inactivation of X-linked inhibitor-of …
XM Sun, SB Bratton, M Butterworth… - Journal of Biological …, 2002 - ASBMB
Bcl-2 and Bcl-x L are reported to inhibit CD95-mediated apoptosis in" type II" but not in" type
I" cells. In the present studies, we found that stimulation of CD95 receptors, with either
agonistic antibody or CD95 ligand, resulted in the activation of caspase-8, which in turn
processed caspase-3 between its large and small subunits. However, in contrast to control
cells, those overexpressing either Bcl-2 or Bcl-x L displayed a distinctive pattern of caspase-
3 processing. Indeed, the resulting p20/p12 caspase-3 was not active and did not undergo …
I" cells. In the present studies, we found that stimulation of CD95 receptors, with either
agonistic antibody or CD95 ligand, resulted in the activation of caspase-8, which in turn
processed caspase-3 between its large and small subunits. However, in contrast to control
cells, those overexpressing either Bcl-2 or Bcl-x L displayed a distinctive pattern of caspase-
3 processing. Indeed, the resulting p20/p12 caspase-3 was not active and did not undergo …