[HTML][HTML] Utilization of sialic acid as a coreceptor enhances reovirus attachment by multistep adhesion strengthening
ES Barton, JL Connolly, JC Forrest, JD Chappell… - Journal of Biological …, 2001 - ASBMB
Many serotype 3 reoviruses bind to two different host cell molecules, sialic acid and an
unidentified protein, using discrete receptor-binding domains in viral attachment protein, ς1.
To determine mechanisms by which these receptor-binding events cooperate to mediate cell
attachment, we generated isogenic reovirus strains that differ in the capacity to bind sialic
acid. Strain SA+, but not SA−, bound specifically to sialic acid on a biosensor chip with
nanomolar avidity. SA+ displayed 5-fold higher avidity for HeLa cells when compared with …
unidentified protein, using discrete receptor-binding domains in viral attachment protein, ς1.
To determine mechanisms by which these receptor-binding events cooperate to mediate cell
attachment, we generated isogenic reovirus strains that differ in the capacity to bind sialic
acid. Strain SA+, but not SA−, bound specifically to sialic acid on a biosensor chip with
nanomolar avidity. SA+ displayed 5-fold higher avidity for HeLa cells when compared with …