[HTML][HTML] A novel fluorescent toxin to detect and investigate Kv1. 3 channel up-regulation in chronically activated T lymphocytes
T lymphocytes with unusually high expression of the voltage-gated Kv1. 3 channel (Kv1. 3
high cells) have been implicated in the pathogenesis of experimental autoimmune
encephalomyelitis, an animal model for multiple sclerosis. We have developed a
fluoresceinated analog of ShK (ShK-F6CA), the most potent known inhibitor of Kv1. 3, for
detection of Kv1. 3 high cells by flow cytometry. ShK-F6CA blocked Kv1. 3 at picomolar
concentrations with a Hill coefficient of 1 and exhibited> 80-fold specificity for Kv1. 3 over …
high cells) have been implicated in the pathogenesis of experimental autoimmune
encephalomyelitis, an animal model for multiple sclerosis. We have developed a
fluoresceinated analog of ShK (ShK-F6CA), the most potent known inhibitor of Kv1. 3, for
detection of Kv1. 3 high cells by flow cytometry. ShK-F6CA blocked Kv1. 3 at picomolar
concentrations with a Hill coefficient of 1 and exhibited> 80-fold specificity for Kv1. 3 over …