Rat adrenal uptake and metabolism of high density lipoprotein cholesteryl ester
JT Gwynne, DD Mahaffee - Journal of Biological Chemistry, 1989 - ASBMB
Metabolism of high density lipoprotein (HDL) cholesteryl ester (CE) by cultured rat adrenal
cells was studied. Addition of [3 H] CE-HDL to cells pretreated with adrenocorticotrophin in
lipoprotein poor media resulted in a time-and concentration-dependent accumulation of [3
H] cholesteryl ester and production of [3 H] cholesterol and [3 H] corticosterone. HDL-CE
metabolism could be described as the sum of a high affinity ([HDL-cholesterol] ½ max= 16
µg/ml) and low affinity ([HDL-cholesterol] ½ max> 70 µg/ml) process.[3 H] Cholesterol was …
cells was studied. Addition of [3 H] CE-HDL to cells pretreated with adrenocorticotrophin in
lipoprotein poor media resulted in a time-and concentration-dependent accumulation of [3
H] cholesteryl ester and production of [3 H] cholesterol and [3 H] corticosterone. HDL-CE
metabolism could be described as the sum of a high affinity ([HDL-cholesterol] ½ max= 16
µg/ml) and low affinity ([HDL-cholesterol] ½ max> 70 µg/ml) process.[3 H] Cholesterol was …