INDIRECTLY PRIMED CD8+ T CELLS ARE A PROMINENT COMPONENT OF THE ALLOGENEIC T-CELL REPERTOIRE AFTER SKIN GRAFT REJECTION IN MICE1
A Valujskikh, C Hartig, PS Heeger - Transplantation, 2001 - journals.lww.com
A Valujskikh, C Hartig, PS Heeger
Transplantation, 2001•journals.lww.comBackground. Alloreactive CD4 and CD8 T lymphocytes recognize antigen through both the
direct and the indirect pathways. Although indirect priming of CD4+ T cells has been well
described, little is known about the frequency and cytokine profile of indirectly primed CD8+
T cells during fully allogeneic graft rejection. Methods. We used a cytokine enzyme-linked
immunosorbent spot assay to characterize indirect priming of alloreactive CD8 and CD4
cells in mice. Results. Interferon-γ-producing CD8+ T cells specific for indirectly presented …
direct and the indirect pathways. Although indirect priming of CD4+ T cells has been well
described, little is known about the frequency and cytokine profile of indirectly primed CD8+
T cells during fully allogeneic graft rejection. Methods. We used a cytokine enzyme-linked
immunosorbent spot assay to characterize indirect priming of alloreactive CD8 and CD4
cells in mice. Results. Interferon-γ-producing CD8+ T cells specific for indirectly presented …
Abstract
Background.
Alloreactive CD4 and CD8 T lymphocytes recognize antigen through both the direct and the indirect pathways. Although indirect priming of CD4+ T cells has been well described, little is known about the frequency and cytokine profile of indirectly primed CD8+ T cells during fully allogeneic graft rejection.
Methods.
We used a cytokine enzyme-linked immunosorbent spot assay to characterize indirect priming of alloreactive CD8 and CD4 cells in mice.
Results.
Interferon-γ-producing CD8+ T cells specific for indirectly presented alloantigen were detectable in allograft-primed mice at a frequency of 50–100 per million cells (compared with 3000 per million for responses through the direct pathway) and were similar in frequency to indirectly primed CD4 cells.
Conclusion.
CD8+ T cells primed through the indirect pathway are a prominent component of the alloreactive T-cell repertoire induced after skin graft placement in mice, raising the possibility that these cells may play a significant role in the rejection process itself.
Lippincott Williams & Wilkins