[6] Correction for liquid junction potentials in patch clamp experiments
E Neher - Methods in enzymology, 1992 - Elsevier
This chapter describes corrections that have to be applied to measured membrane
potentials in patch clamp experiments. Some of them [Eqs.(1)–(3)] are required regardless of
the nature of the reference electrode (in the Ringer's solution bath) whenever the pipette-
filling solution is different from the bath solution. They represent the liquid junction potentials
that are present at the pipette tip before patch formation. In addition, corrections have to be
applied when the bath solution is being changed during a measurement (ie, after seal …
potentials in patch clamp experiments. Some of them [Eqs.(1)–(3)] are required regardless of
the nature of the reference electrode (in the Ringer's solution bath) whenever the pipette-
filling solution is different from the bath solution. They represent the liquid junction potentials
that are present at the pipette tip before patch formation. In addition, corrections have to be
applied when the bath solution is being changed during a measurement (ie, after seal …