[HTML][HTML] Molecular uncoupling of fractalkine-mediated cell adhesion and signal transduction: rapid flow arrest of CX3CR1-expressing cells is independent of G-protein …
CA Haskell, MD Cleary, IF Charo - Journal of Biological Chemistry, 1999 - ASBMB
Fractalkine is a novel multidomain protein expressed on the surface of activated endothelial
cells. Cells expressing the chemokine receptor CX 3 CR1 adhere to fractalkine with high
affinity, but it is not known if adherence requires G-protein activation and signal transduction.
To investigate the cell adhesion properties of fractalkine, we created mutated forms of CX 3
CR1 that have little or no ability to transduce intracellular signals. Cells expressing signaling-
incompetent forms of CX 3 CR1 bound rapidly and with high affinity to immobilized …
cells. Cells expressing the chemokine receptor CX 3 CR1 adhere to fractalkine with high
affinity, but it is not known if adherence requires G-protein activation and signal transduction.
To investigate the cell adhesion properties of fractalkine, we created mutated forms of CX 3
CR1 that have little or no ability to transduce intracellular signals. Cells expressing signaling-
incompetent forms of CX 3 CR1 bound rapidly and with high affinity to immobilized …