[HTML][HTML] L-ascorbic acid potentiates endothelial nitric oxide synthesis via a chemical stabilization of tetrahydrobiopterin
R Heller, A Unbehaun, B Schellenberg, B Mayer… - Journal of biological …, 2001 - ASBMB
Ascorbic acid has been shown to stimulate endothelial nitric oxide (NO) synthesis in a time-
and concentration-dependent fashion without affecting NO synthase (NOS) expression or l-
arginine uptake. The present study investigates if the underlying mechanism is related to the
NOS cofactor tetrahydrobiopterin. Pretreatment of human umbilical vein endothelial cells
with ascorbate (1 μm to 1 mm, 24 h) led to an up to 3-fold increase of intracellular
tetrahydrobiopterin levels that was concentration-dependent and saturable at 100 μm …
and concentration-dependent fashion without affecting NO synthase (NOS) expression or l-
arginine uptake. The present study investigates if the underlying mechanism is related to the
NOS cofactor tetrahydrobiopterin. Pretreatment of human umbilical vein endothelial cells
with ascorbate (1 μm to 1 mm, 24 h) led to an up to 3-fold increase of intracellular
tetrahydrobiopterin levels that was concentration-dependent and saturable at 100 μm …