[HTML][HTML] Quantitative Assessment of Gene Targeting in Vitroand in Vivo by the Pancreatic Transcription Factor, Pdx1: IMPORTANCE OF CHROMATIN STRUCTURE IN …
SK Chakrabarti, JC James, RG Mirmira - Journal of Biological Chemistry, 2002 - ASBMB
The transcription factor Pdx1 is expressed in the pancreatic β-cell, where it is believed to
regulate several β-cell-specific genes. Whereas binding by Pdx1 to elements of β-cell genes
has been demonstrated in vitro, almost none of these genes has been demonstrated to be a
direct binding target for Pdx1 within cells (where complex chromatin structure exists). To
determine which β-cell promoters are bound by Pdx1 in vivo, we performed chromatin
immunoprecipitation assays using Pdx1 antiserum and chromatin from β-TC3 cells and …
regulate several β-cell-specific genes. Whereas binding by Pdx1 to elements of β-cell genes
has been demonstrated in vitro, almost none of these genes has been demonstrated to be a
direct binding target for Pdx1 within cells (where complex chromatin structure exists). To
determine which β-cell promoters are bound by Pdx1 in vivo, we performed chromatin
immunoprecipitation assays using Pdx1 antiserum and chromatin from β-TC3 cells and …