[HTML][HTML] Co-localization of Leukotriene A4Hydrolase with 5-Lipoxygenase in Nuclei of Alveolar Macrophages and Rat Basophilic Leukemia Cells but Not Neutrophils
TG Brock, E Maydanski, RW McNish… - Journal of Biological …, 2001 - ASBMB
The synthesis of leukotriene B 4 from arachidonic acid requires the sequential action of two
enzymes: 5-lipoxygenase and leukotriene A 4 hydrolase. 5-Lipoxygenase is known to be
present in the cytoplasm of some leukocytes and able to accumulate in the nucleoplasm of
others. In this study, we asked if leukotriene A 4 hydrolase co-localizes with 5-lipoxygenase
in different types of leukocytes. Examination of rat basophilic leukemia cells by both
immunocytochemistry and immunofluorescence revealed that leukotriene A 4 hydrolase, like …
enzymes: 5-lipoxygenase and leukotriene A 4 hydrolase. 5-Lipoxygenase is known to be
present in the cytoplasm of some leukocytes and able to accumulate in the nucleoplasm of
others. In this study, we asked if leukotriene A 4 hydrolase co-localizes with 5-lipoxygenase
in different types of leukocytes. Examination of rat basophilic leukemia cells by both
immunocytochemistry and immunofluorescence revealed that leukotriene A 4 hydrolase, like …