[HTML][HTML] 6-O-sulfotransferase-1 represents a critical enzyme in the anticoagulant heparan sulfate biosynthetic pathway
Using recombinant retroviral transduction, we have introduced the heparin/heparan sulfate
(HS) 3-O-sulfotransferase 1 (3-OST-1) gene into Chinese hamster ovary (CHO) cells.
Expression of 3-OST-1confers upon CHO cells the ability to produce anticoagulantly active
HS (HS act). To understand how 6-OST and other proteins regulate HS act biosynthesis, a
CHO cell clone with three copies of 3-OST-1 was chemically mutagenized. Resulting
mutants that make HS but are defective in generating HS act were single-cell-cloned. One …
(HS) 3-O-sulfotransferase 1 (3-OST-1) gene into Chinese hamster ovary (CHO) cells.
Expression of 3-OST-1confers upon CHO cells the ability to produce anticoagulantly active
HS (HS act). To understand how 6-OST and other proteins regulate HS act biosynthesis, a
CHO cell clone with three copies of 3-OST-1 was chemically mutagenized. Resulting
mutants that make HS but are defective in generating HS act were single-cell-cloned. One …