[HTML][HTML] Multiple isoforms of heparan sulfate D-glucosaminyl 3-O-sulfotransferase: isolation, characterization, and expression of human cDNAs and identification of …
3-O-Sulfated glucosaminyl residues are rare constituents of heparan sulfate and are
essential for the activity of anticoagulant heparan sulfate. Cellular production of the critical
active structure is controlled by the rate-limiting enzyme, heparan sulfate d-glucosaminyl 3-O-
sulfotransferase-1 (3-OST-1)(EC 2.8. 2.23). We have probed the expressed sequence tag
data base with the carboxyl-terminal sulfotransferase domain of 3-OST-1 to reveal three
novel, incomplete human cDNAs. These were utilized in library screens to isolate full-length …
essential for the activity of anticoagulant heparan sulfate. Cellular production of the critical
active structure is controlled by the rate-limiting enzyme, heparan sulfate d-glucosaminyl 3-O-
sulfotransferase-1 (3-OST-1)(EC 2.8. 2.23). We have probed the expressed sequence tag
data base with the carboxyl-terminal sulfotransferase domain of 3-OST-1 to reveal three
novel, incomplete human cDNAs. These were utilized in library screens to isolate full-length …