[HTML][HTML] Purification of heparan sulfate D-glucosaminyl 3-O-sulfotransferase
The cellular generation of proteoglycans with anticoagulant heparan sulfate (HSPG act) is
determined by microsomal" HS act conversion activity" that functions in concert with the
sulfate donor 3′-phosphoadenosine 5′-phosphosulfate (PAPS) to convert
nonanticoagulant heparan sulfate (HS inact) to anticoagulant heparan sulfate (HS
act)(Shworak, NW, Fritze, LMS, Liu, J., Butler, LD, and Rosenberg, RD (1996) J. Biol. Chem.
271, 27063-27071). Suspension cultures of L-33+ cells in serum-free medium produce …
determined by microsomal" HS act conversion activity" that functions in concert with the
sulfate donor 3′-phosphoadenosine 5′-phosphosulfate (PAPS) to convert
nonanticoagulant heparan sulfate (HS inact) to anticoagulant heparan sulfate (HS
act)(Shworak, NW, Fritze, LMS, Liu, J., Butler, LD, and Rosenberg, RD (1996) J. Biol. Chem.
271, 27063-27071). Suspension cultures of L-33+ cells in serum-free medium produce …