Recently, there have been several reports demonstrating improvements in the flow
cytometric detection of intracellular cytokines. These advances, although significant, have
not yielded techniques that have easily been translated into broad use. To address this
issue, we have coupled a fixation and permeabilization method with the use of directly
labelled monoclonal anti-cytokine antibodies, providing both improved signal and simpler
staining. The kinetics of in situ cytokine production in both CD4 and CD8 cells are shown for …