New fluorescent dyes for lymphocyte migration studies: analysis by flow cytometry and fluorescence microscopy

SA Weston, CR Parish - Journal of immunological methods, 1990 - Elsevier
Abstract 16 fluorochromes were examined for their ability to label viable lymphocytes in vitro
and yield fluorescence detectable by fluorescence microscopy and flow cytometry. Of these
fluorochromes, four intracellular dyes were found to be suitable for in vivo migration studies.
They were H33342, the well known DNA-binding dye which excites and emits in the UV
range, and three fluorescein based cytoplasmic dyes, namely BCECF-AM, Calcein-AM and
CFSE which excite and emit in the visible range. Lymphocytes labelled with H33342 …