[27] Isolation of human cell lines lacking mitochondrial DNA

MP King, G Attardi - Methods in enzymology, 1996 - Elsevier
Publisher Summary This chapter discusses isolation of human cell lines lacking
mitochondrial DNA. An understanding of the molecular genetic mechanisms by which these
mutations act can provide insights into the etiology, pathogenesis, and ultimately the
treatment of these diseases, and may enhance the knowledge of mitochondrial biogenesis.
For investigating nucleomitochondrial interactions and the molecular pathogenetic
mechanisms of mtDNA mutations, it is useful to manipulate the mtDNA complement of a cell …