Molecular cloning of a putative Ca2+-sensing receptor cDNA from human kidney
K Aida, S Koishi, M Tawata, T Onaya - Biochemical and biophysical …, 1995 - Elsevier
K Aida, S Koishi, M Tawata, T Onaya
Biochemical and biophysical research communications, 1995•ElsevierA cDNA that encodes a putative Ca2+-sensing receptor (HuKCaSR) was cloned from
human kidney with the use of the polymerase chain reaction. The predicted HuKCaSR
protein consists of 1078 amino acids and shares 93.1 and 93.8% overall identity with the
bovine parathyroid Ca2+-sensing receptor (BoPCaR1) and rat kidney Ca2+-sensing
receptor (RaKCaR), respectively, with least similarity in the carboxyl-terminal regions. The
HuKCaSR gene was mapped by fluorescence in situ hybridization to chromosome 3q21, at …
human kidney with the use of the polymerase chain reaction. The predicted HuKCaSR
protein consists of 1078 amino acids and shares 93.1 and 93.8% overall identity with the
bovine parathyroid Ca2+-sensing receptor (BoPCaR1) and rat kidney Ca2+-sensing
receptor (RaKCaR), respectively, with least similarity in the carboxyl-terminal regions. The
HuKCaSR gene was mapped by fluorescence in situ hybridization to chromosome 3q21, at …
A cDNA that encodes a putative Ca2+-sensing receptor (HuKCaSR) was cloned from human kidney with the use of the polymerase chain reaction. The predicted HuKCaSR protein consists of 1078 amino acids and shares 93.1 and 93.8% overall identity with the bovine parathyroid Ca2+-sensing receptor (BoPCaR1) and rat kidney Ca2+-sensing receptor (RaKCaR), respectively, with least similarity in the carboxyl-terminal regions. The HuKCaSR gene was mapped by fluorescence in situ hybridization to chromosome 3q21, at which region the gene responsible for familial hypocalciuric hypercalcemia has previously been localized by genetic linkage analysis. RNA blot analysis revealed HuKCaSR mRNA in human kidney, but not in brain, lung, liver, heart, skeletal muscle, or placenta.
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