[HTML][HTML] Maximal inhibition of SERCA2 Ca2+ affinity by phospholamban in transgenic hearts overexpressing a non-phosphorylatable form of phospholamban
AG Brittsan, AN Carr, AG Schmidt… - Journal of Biological …, 2000 - ASBMB
Phospholamban is a phosphoprotein in the cardiac sarcoplasmic reticulum (SR) which
regulates the apparent Ca 2+ affinity of the SR Ca 2+-ATPase (SERCA2). To determine the
levels of phospholamban which are associated with maximal inhibition of SERCA2, several
lines of transgenic mice were generated which expressed increasing levels of a non-
phosphorylatable form of phospholamban (S16A, T17A) specifically in the heart. This mutant
form of phospholamban was chosen to prevent phosphorylation as a compensatory …
regulates the apparent Ca 2+ affinity of the SR Ca 2+-ATPase (SERCA2). To determine the
levels of phospholamban which are associated with maximal inhibition of SERCA2, several
lines of transgenic mice were generated which expressed increasing levels of a non-
phosphorylatable form of phospholamban (S16A, T17A) specifically in the heart. This mutant
form of phospholamban was chosen to prevent phosphorylation as a compensatory …