Reduction of [Ca2+]i Restores Uncoupled β-Adrenergic Signaling in Isolated Perfused Transgenic Mouse Hearts
VB Serikov, NN Petrashevskaya, AM Canning… - Circulation …, 2001 - Am Heart Assoc
VB Serikov, NN Petrashevskaya, AM Canning, A Schwartz
Circulation research, 2001•Am Heart AssocThe effects of alterations in calcium in the perfusion media were studied on β-adrenergic
coupling in isolated hearts from 3 different transgenic mice: cardiac-specific overexpressed
α 1 subunit of L-type calcium channel, overexpressed Gα q, and phospholamban knockout.
Isolated hearts from all 3 models, when studied at [Ca 2+] of 2 mmol/L in the perfusate,
showed the usual blunted or no response to β-adrenergic stimulation. Lowering [Ca 2+] to
0.75 to 1.5 mmol/L unloaded the hearts of calcium and restored to nearly normal the …
coupling in isolated hearts from 3 different transgenic mice: cardiac-specific overexpressed
α 1 subunit of L-type calcium channel, overexpressed Gα q, and phospholamban knockout.
Isolated hearts from all 3 models, when studied at [Ca 2+] of 2 mmol/L in the perfusate,
showed the usual blunted or no response to β-adrenergic stimulation. Lowering [Ca 2+] to
0.75 to 1.5 mmol/L unloaded the hearts of calcium and restored to nearly normal the …
The effects of alterations in calcium in the perfusion media were studied on β-adrenergic coupling in isolated hearts from 3 different transgenic mice: cardiac-specific overexpressed α 1 subunit of L-type calcium channel, overexpressed Gα q , and phospholamban knockout. Isolated hearts from all 3 models, when studied at [Ca 2+ ] of 2 mmol/L in the perfusate, showed the usual blunted or no response to β-adrenergic stimulation. Lowering [Ca 2+ ] to 0.75 to 1.5 mmol/L unloaded the hearts of calcium and restored to nearly normal the responsiveness to β-agonist stimulation.
Am Heart Assoc