Prodrug-activated gene therapy: involvement of an immunological component in the" bystander effect".
S Gagandeep, R Brew, B Green, SE Christmas… - Cancer gene …, 1996 - europepmc.org
Cancer gene therapy, 1996•europepmc.org
The integration and expression of the herpes simplex virus type 1 thymidine kinase (HSV1-
TK) gene in localized tumors results in tumor regression after the administration of the
specific nucleoside analogue ganciclovir (GCV). Although only 10% to 20% of the tumor
cells take up the HSV1-TK gene, the neighboring cells also die, a phenomenon termed"
bystander effect.". In the present study, coinjection of the MC26 mouse colon carcinoma cell
line and the HSV1-TK expressing retroviral packaging cell line followed after 7 days by the …
TK) gene in localized tumors results in tumor regression after the administration of the
specific nucleoside analogue ganciclovir (GCV). Although only 10% to 20% of the tumor
cells take up the HSV1-TK gene, the neighboring cells also die, a phenomenon termed"
bystander effect.". In the present study, coinjection of the MC26 mouse colon carcinoma cell
line and the HSV1-TK expressing retroviral packaging cell line followed after 7 days by the …
The integration and expression of the herpes simplex virus type 1 thymidine kinase (HSV1-TK) gene in localized tumors results in tumor regression after the administration of the specific nucleoside analogue ganciclovir (GCV). Although only 10% to 20% of the tumor cells take up the HSV1-TK gene, the neighboring cells also die, a phenomenon termed" bystander effect.". In the present study, coinjection of the MC26 mouse colon carcinoma cell line and the HSV1-TK expressing retroviral packaging cell line followed after 7 days by the intraperitoneal administration of GCV resulted in almost total tumor regression in the immunocompetent BALB/c mice but not in immunocompromised athymic BALB/c mice. This suggested a strong cell-mediated immune component to the bystander effect.
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