[HTML][HTML] Mass spectrometric analysis of nitric oxide-modified caspase-3
B Zech, M Wilm, R van Eldik, B Brune - Journal of Biological Chemistry, 1999 - ASBMB
Caspases are a family of cysteine proteases activated during apoptosis. Modification of
caspases by nitric oxide and its relevance during apoptosis is currently a controversial
subject. In this study we analyzed the S-nitrosated form of caspase-3 at a molecular level. By
using electrospray ionization-mass spectrometry, we detected poly-S-nitrosation of caspase-
3 with an average of about 2 molecules of NO bound per enzyme. Although NO treatment
completely inhibited enzyme activity, S-nitrosation was not restricted to the active site …
caspases by nitric oxide and its relevance during apoptosis is currently a controversial
subject. In this study we analyzed the S-nitrosated form of caspase-3 at a molecular level. By
using electrospray ionization-mass spectrometry, we detected poly-S-nitrosation of caspase-
3 with an average of about 2 molecules of NO bound per enzyme. Although NO treatment
completely inhibited enzyme activity, S-nitrosation was not restricted to the active site …
