Nitric oxide inhibits caspase-3 by S-nitrosationin vivo
L Rössig, B Fichtlscherer, K Breitschopf… - Journal of Biological …, 1999 - ASBMB
In cultured human endothelial cells, physiological levels of NO prevent apoptosis and
interfere with the activation of the caspase cascade. In vitro data have demonstrated that NO
inhibits the activity of caspase-3 byS-nitrosation of the enzyme. Here we present evidence
for the in vivo occurrence and functional relevance of this novel antiapoptotic mechanism. To
demonstrate that the cysteine residue Cys-163 of caspase-3 is S-nitrosated, cells were
transfected with the Myc-tagged p17 subunit of caspase-3. After incubation of the transfected …
interfere with the activation of the caspase cascade. In vitro data have demonstrated that NO
inhibits the activity of caspase-3 byS-nitrosation of the enzyme. Here we present evidence
for the in vivo occurrence and functional relevance of this novel antiapoptotic mechanism. To
demonstrate that the cysteine residue Cys-163 of caspase-3 is S-nitrosated, cells were
transfected with the Myc-tagged p17 subunit of caspase-3. After incubation of the transfected …
