[HTML][HTML] Insulin-stimulated hydrogen peroxide reversibly inhibits protein-tyrosine phosphatase 1b in vivo and enhances the early insulin action cascade
K Mahadev, A Zilbering, L Zhu, BJ Goldstein - Journal of Biological …, 2001 - ASBMB
The insulin signaling pathway is activated by tyrosine phosphorylation of the insulin receptor
and key post-receptor substrate proteins and balanced by the action of specific protein-
tyrosine phosphatases (PTPases). PTPase activity, in turn, is highly regulated in vivo by
oxidation/reduction reactions involving the cysteine thiol moiety required for catalysis. Here
we show that insulin stimulation generates a burst of intracellular H 2 O 2 in insulin-sensitive
hepatoma and adipose cells that is associated with reversible oxidative inhibition of up to …
and key post-receptor substrate proteins and balanced by the action of specific protein-
tyrosine phosphatases (PTPases). PTPase activity, in turn, is highly regulated in vivo by
oxidation/reduction reactions involving the cysteine thiol moiety required for catalysis. Here
we show that insulin stimulation generates a burst of intracellular H 2 O 2 in insulin-sensitive
hepatoma and adipose cells that is associated with reversible oxidative inhibition of up to …