Miniature endplate current rise times less than 100 microseconds from improved dual recordings can be modeled with passive acetylcholine diffusion from a synaptic …
JR Stiles, D Van Helden, TM Bartol Jr… - Proceedings of the …, 1996 - National Acad Sciences
We recorded miniature endplate currents (mEPCs) using simultaneous voltage clamp and
extracellular methods, allowing correction for time course measurement errors. We obtained
a 20-80% rise time (tr) of approximately 80 micros at 22 degrees C, shorter than any
previously reported values, and tr variability (SD) with an upper limit of 25-30 micros.
Extracellular electrode pressure can increase tr and its variability by 2-to 3-fold. Using Monte
Carlo simulations, we modeled passive acetylcholine diffusion through a vesicle fusion pore …
extracellular methods, allowing correction for time course measurement errors. We obtained
a 20-80% rise time (tr) of approximately 80 micros at 22 degrees C, shorter than any
previously reported values, and tr variability (SD) with an upper limit of 25-30 micros.
Extracellular electrode pressure can increase tr and its variability by 2-to 3-fold. Using Monte
Carlo simulations, we modeled passive acetylcholine diffusion through a vesicle fusion pore …
Miniature endplate current rise times< 100 μ s from improved dual recordings can be modeled with passive acetylcholine diffusion from a synaptic vesicle
JR Stiles, D Van Helden, TM Bartol, EE Salpeter… - Proceedings of the …, 1996 - JSTOR
We recorded miniature endplate currents (mEPCs) using simultaneous voltage clamp and
extracellular methods, allowing correction for time course measurement errors. We obtained
a 20-80% rise time (tr) of≈ 80 μ s at 22 degrees C, shorter than any previously reported
values, and tr variability (SD) with an upper limit of 25-30 μ s. Extracellular electrode
pressure can increase tr and its variability by 2-to 3-fold. Using Monte Carlo simulations, we
modeled passive acetylcholine diffusion through a vesicle fusion pore expanding radially at …
extracellular methods, allowing correction for time course measurement errors. We obtained
a 20-80% rise time (tr) of≈ 80 μ s at 22 degrees C, shorter than any previously reported
values, and tr variability (SD) with an upper limit of 25-30 μ s. Extracellular electrode
pressure can increase tr and its variability by 2-to 3-fold. Using Monte Carlo simulations, we
modeled passive acetylcholine diffusion through a vesicle fusion pore expanding radially at …