Expression of functional acetylcholine receptor from cloned cDNAs
M Mishina, T Kurosaki, T Tobimatsu, Y Morimoto… - Nature, 1984 - nature.com
The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine
receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional
receptor in a combined expression system consisting of COS monkey cells and Xenopus
oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic
response to acetylcholine, whereas only the α-subunit is indispensable for α-bungarotoxin
binding activity.
receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional
receptor in a combined expression system consisting of COS monkey cells and Xenopus
oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic
response to acetylcholine, whereas only the α-subunit is indispensable for α-bungarotoxin
binding activity.
Abstract
The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the α-subunit is indispensable for α-bungarotoxin binding activity.
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