Location of functional regions of acetylcholine receptor α-subunit by site-directed mutagenesis
M Mishina, T Tobimatsu, K Imoto, K Tanaka, Y Fujita… - Nature, 1985 - nature.com
M Mishina, T Tobimatsu, K Imoto, K Tanaka, Y Fujita, K Fukuda, M Kurasaki, H Takahashi…
Nature, 1985•nature.comThe availability of cloned cDNAs encoding the four subunits of the Torpedo acetylcholine
receptor, which can be expressed to make functional receptors in Xenopus oocytes, has
made possible a detailed investigation of the functions of the different structural components
of the receptor. The functional analysis of receptors with α-subunits altered at specific sites
by site-directed mutagenesis of the cDNA has allowed the location of specific regions of the
α-subunit molecule involved in acetylcholine binding and forming a transmembrane ionic …
receptor, which can be expressed to make functional receptors in Xenopus oocytes, has
made possible a detailed investigation of the functions of the different structural components
of the receptor. The functional analysis of receptors with α-subunits altered at specific sites
by site-directed mutagenesis of the cDNA has allowed the location of specific regions of the
α-subunit molecule involved in acetylcholine binding and forming a transmembrane ionic …
Abstract
The availability of cloned cDNAs encoding the four subunits of the Torpedo acetylcholine receptor, which can be expressed to make functional receptors in Xenopus oocytes, has made possible a detailed investigation of the functions of the different structural components of the receptor. The functional analysis of receptors with α-subunits altered at specific sites by site-directed mutagenesis of the cDNA has allowed the location of specific regions of the α-subunit molecule involved in acetylcholine binding and forming a transmembrane ionic channel.
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