[HTML][HTML] Transcriptional control of the pref-1 gene in 3T3-L1 adipocyte differentiation: sequence requirement for differentiation-dependent suppression
CM Smas, D Kachinskas, CM Liu, X Xie… - Journal of biological …, 1998 - ASBMB
Preadipocyte factor-1 (Pref-1) is a transmembrane epidermal growth factor-like domain-
containing protein highly expressed in 3T3-L1 preadipocytes, but is undetectable in mature
fat cells; this down-regulation is required for adipocyte differentiation. We show here that pref-
1 transcription is markedly suppressed during adipose conversion and results in decreased
Pref-1 RNA levels. Using 3T3-L1 cells stably transfected with Pref-1 5′-deletion constructs
truncated at− 6000,− 2100,− 1300,− 692,− 300,− 235,− 193,− 183,− 170,− 93, and− 45 base …
containing protein highly expressed in 3T3-L1 preadipocytes, but is undetectable in mature
fat cells; this down-regulation is required for adipocyte differentiation. We show here that pref-
1 transcription is markedly suppressed during adipose conversion and results in decreased
Pref-1 RNA levels. Using 3T3-L1 cells stably transfected with Pref-1 5′-deletion constructs
truncated at− 6000,− 2100,− 1300,− 692,− 300,− 235,− 193,− 183,− 170,− 93, and− 45 base …