Adipocytes contain large lipid droplets in their cytoplasm. When cultured, they float on top of
the medium, clump together, and do not gain equal and sufficient access to the medium.
Morphological changes cannot be observed and the majority of adipocytes undergo cell
lysis within 72 h of isolation. We have used a ceiling culture method for human mature
adipocytes which uses their buoyant property to allow them to adhere to a floating glass
surface, where they remain viable for several weeks. Using confocal immunofluorescence …