Ca2+-mobilising agonists potentiate forskolin-and VIP-stimulated cAMP production in human colonic cell line, HT29-cl. 19A: Role of [Ca2+] i and protein kinase C
G Warhurst, KE Fogg, NB Higgs, A Tonge, J Grundy - Cell calcium, 1994 - Elsevier
This study has examined the involvement of the Ca 2+-signalling pathway in the regulation
of agonist-stimulated cAMP responses in the human colonic adenocarcinoma cell line, HT29-
cl. 19A. The muscarinic agonist, carbachol (CCh) stimulated rapid increases in cellular IP 3
and cytosolic Ca 2+,[Ca 2+] i in HT29-cl. 19A cells. These were accompanied by a small but
significant increase in basal cAMP levels and a marked (3–4-fold) potentiation of both
forskolin-(FSK) and VIP-stimulated cAMP generation. Similar effects were observed with two …
of agonist-stimulated cAMP responses in the human colonic adenocarcinoma cell line, HT29-
cl. 19A. The muscarinic agonist, carbachol (CCh) stimulated rapid increases in cellular IP 3
and cytosolic Ca 2+,[Ca 2+] i in HT29-cl. 19A cells. These were accompanied by a small but
significant increase in basal cAMP levels and a marked (3–4-fold) potentiation of both
forskolin-(FSK) and VIP-stimulated cAMP generation. Similar effects were observed with two …