Time resolved absorption study of the reaction of hydroxyurea with sickle cell hemoglobin
Biochimica et Biophysica Acta (BBA)-General Subjects, 1998•Elsevier
Hydroxyurea has been mixed with hemoglobin S and the reaction was studied using
electronic absorption spectroscopy as a function of time and wavelength. The rate of
conversion of oxyhemoglobin S to other species was determined and the nature of the
reaction products was studied. We also report the formation of methemoglobin (and other
reaction products) when deoxyhemoglobin S is combined with hydroxyurea. The probable
increase in the formation of methemoglobin, and other potential reaction products such as …
electronic absorption spectroscopy as a function of time and wavelength. The rate of
conversion of oxyhemoglobin S to other species was determined and the nature of the
reaction products was studied. We also report the formation of methemoglobin (and other
reaction products) when deoxyhemoglobin S is combined with hydroxyurea. The probable
increase in the formation of methemoglobin, and other potential reaction products such as …
Hydroxyurea has been mixed with hemoglobin S and the reaction was studied using electronic absorption spectroscopy as a function of time and wavelength. The rate of conversion of oxyhemoglobin S to other species was determined and the nature of the reaction products was studied. We also report the formation of methemoglobin (and other reaction products) when deoxyhemoglobin S is combined with hydroxyurea. The probable increase in the formation of methemoglobin, and other potential reaction products such as nitric oxide-hemoglobin, in patients with sickle cell anemia who are taking hydroxyurea as a therapeutic drug is discussed in terms of the pathophysiology of the disease. It is proposed that methemoglobin and possibly nitric oxide-hemoglobin formation may partially explain beneficial effects observed in these patients before their levels of fetal hemoglobin have increased.
Elsevier