Identification of type II collagen peptide 261–273‐specific T cell clones in a patient with relapsing polychondritis
JH Buckner, M Van Landeghen, WW Kwok… - Arthritis & …, 2002 - Wiley Online Library
JH Buckner, M Van Landeghen, WW Kwok, L Tsarknaridis
Arthritis & Rheumatism, 2002•Wiley Online LibraryObjective To characterize and clone T cells specific for type II collagen (CII) in a patient with
relapsing polychondritis (RP) and to establish whether the immunodominant epitope of CII
determined in HLA transgenic mice is used in the human autoimmune response to CII.
Methods T cell responses to CII were examined in a patient with RP, who was heterozygous
for the HLA‐DR allele DRB1* 0101/DRB1* 0401. T cell clones were established from this
patient and characterized for peptide specificity, class II restriction, cytokine production, and …
relapsing polychondritis (RP) and to establish whether the immunodominant epitope of CII
determined in HLA transgenic mice is used in the human autoimmune response to CII.
Methods T cell responses to CII were examined in a patient with RP, who was heterozygous
for the HLA‐DR allele DRB1* 0101/DRB1* 0401. T cell clones were established from this
patient and characterized for peptide specificity, class II restriction, cytokine production, and …
Objective
To characterize and clone T cells specific for type II collagen (CII) in a patient with relapsing polychondritis (RP) and to establish whether the immunodominant epitope of CII determined in HLA transgenic mice is used in the human autoimmune response to CII.
Methods
T cell responses to CII were examined in a patient with RP, who was heterozygous for the HLA‐DR allele DRB1*0101/DRB1*0401. T cell clones were established from this patient and characterized for peptide specificity, class II restriction, cytokine production, and staining with HLA‐DRB1*0401 class II tetramers.
Results
A response to CII and the peptide 255–273 was present in this patient. T cells specific for the CII epitope 261–273 were cloned. Evaluation of these clones demonstrated a response to CII 261–273 in the context of both DR alleles. HLA‐DR4 CII tetramer did not demonstrate staining of either CII‐specific DRB1*0401‐restricted T cell clones or a polyclonal population of CII‐reactive T cells from this individual.
Conclusion
T cells directed against CII were present in this patient with RP. Also, T cell clones isolated from this individual were found to be specific for the CII peptide 261–273 and were restricted to either the DRB1*0101 or the DRB1*0401 allele. These findings establish that a T cell response directed against CII is present in this patient with RP and that the CII peptide 261–273 plays a role in the human immune response to CII.
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