Reduced glutathione depletion causes necrosis and sensitization to tumor necrosis factor-α-induced apoptosis in cultured mouse hepatocytes
H Nagai, K Matsumaru, G Feng, N Kaplowitz - Hepatology, 2002 - journals.lww.com
The effect of reduced glutathione (GSH) depletion by acetaminophen (APAP),
diethylmaleate (DEM), or phorone on the mode of cell death and susceptibility to tumor
necrosis factor (TNF)-induced cell death was studied in cultured mouse hepatocytes. Dose-
dependent necrosis was the exclusive mode of cell death with APAP alone, but the addition
of TNF-α induced a switch to about half apoptosis without changing total loss of viability. This
effect was seen at 1 and 5 mmol/L but was inhibited at 10 and 20 mmol/L APAP. The switch …
diethylmaleate (DEM), or phorone on the mode of cell death and susceptibility to tumor
necrosis factor (TNF)-induced cell death was studied in cultured mouse hepatocytes. Dose-
dependent necrosis was the exclusive mode of cell death with APAP alone, but the addition
of TNF-α induced a switch to about half apoptosis without changing total loss of viability. This
effect was seen at 1 and 5 mmol/L but was inhibited at 10 and 20 mmol/L APAP. The switch …