[HTML][HTML] Sphingolipid Biosynthesis de Novo by Rat Hepatocytes in Culture.: CERAMIDE AND SPHINGOMYELIN ARE ASSOCIATED WITH, BUT NOT REQUIRED FOR …
AH Merrill, S Lingrell, E Wang… - Journal of Biological …, 1995 - ASBMB
Sphingolipids are constituents of liver and lipoproteins, but relatively little is known about
their synthesis and secretion. Incubation of rat hepatocytes with [14 C]-or [3 H] serine
labeled the long-chain base backbones of mainly ceramide and sphingomyelin. Most of the
labeled sphingolipids were associated with the cells; however, 1-5%(the majority of which
was ceramide) was released into the medium as part of very low density lipoproteins
(VLDL). Since this is the first report that lipoproteins contain ceramide, lipoproteins were …
their synthesis and secretion. Incubation of rat hepatocytes with [14 C]-or [3 H] serine
labeled the long-chain base backbones of mainly ceramide and sphingomyelin. Most of the
labeled sphingolipids were associated with the cells; however, 1-5%(the majority of which
was ceramide) was released into the medium as part of very low density lipoproteins
(VLDL). Since this is the first report that lipoproteins contain ceramide, lipoproteins were …