Epinephrine and norepinephrine binding sites with the physiological characteristics of alpha-adrenergic receptors have been identified in the plasma membranes of liver and other cells. Interaction of catecholamines with these receptors causes a mobilization of calcium ions from mitochondria and perhaps other intracellular stores in liver cells. In other cells, there may also be influx of extracellular calcium ions. Evidence is presented in support of the hypothesis that the rise in cytosolic calcium ions resulting from these changes is responsible for many of the alpha-adrenergic actions of catecholamines. Possible mechanisms by which activation of alpha-adrenergic receptors causes changes in calcium and other aspects of cellular metabolism are discussed.