Coupling of the TCR to integrin activation by Slap-130/Fyb

EJ Peterson, ML Woods, SA Dmowski, G Derimanov… - Science, 2001 - science.org
EJ Peterson, ML Woods, SA Dmowski, G Derimanov, MS Jordan, JN Wu, PS Myung, QH Liu…
Science, 2001science.org
SLAP-130/Fyb (SLP-76–associated phosphoprotein or Fyn-binding protein; also known as
Fyb/Slap) is a hematopoietic-specific adapter, which associates with and modulates function
of SH2-containing leukocyte phosphoprotein of 76 kilodaltons (SLP-76). T cells from mice
lacking SLAP-130/Fyb show markedly impaired proliferation following CD3 engagement. In
addition, the T cell receptor (TCR) in SLAP-130/Fyb mutant cells fails to enhance integrin-
dependent adhesion. Although TCR-induced actin polymerization is normal, TCR-stimulated …
SLAP-130/Fyb (SLP-76–associated phosphoprotein or Fyn-binding protein; also known as Fyb/Slap) is a hematopoietic-specific adapter, which associates with and modulates function of SH2-containing leukocyte phosphoprotein of 76 kilodaltons (SLP-76). T cells from mice lacking SLAP-130/Fyb show markedly impaired proliferation following CD3 engagement. In addition, the T cell receptor (TCR) in SLAP-130/Fyb mutant cells fails to enhance integrin-dependent adhesion. Although TCR-induced actin polymerization is normal, TCR-stimulated clustering of the integrin LFA-1 is defective in SLAP-130/Fyb–deficient cells. These data indicate that SLAP-130/Fyb is important for coupling TCR-mediated actin cytoskeletal rearrangement with activation of integrin function, and for T cells to respond fully to activating signals.
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