Expression cloning of an adenylate cyclase-coupled calcitonin receptor

HY Lin, TL Harris, MS Flannery, A Aruffo, EH Kaji… - Science, 1991 - science.org
HY Lin, TL Harris, MS Flannery, A Aruffo, EH Kaji, A Gorn, LF Kolakowski Jr, HF Lodish…
Science, 1991science.org
A calcitonin receptor complementary DNA (cDNA) was cloned by expression of a cDNA
library from a porcine kidney epithelial cell line in COS cells. The 482-amino acid receptor
has high affinity for salmon calcitonin (dissociation constant K d≈ 6 nM) and is functionally
coupled to increases in intracellular cyclic adenosine monophosphate (cAMP). The receptor
shows no sequence similarity to other reported G protein-coupled receptors but is
homologous to the parathyroid hormone-parathyroid hormone-related peptide (PTH-PTHrP) …
A calcitonin receptor complementary DNA (cDNA) was cloned by expression of a cDNA library from a porcine kidney epithelial cell line in COS cells. The 482-amino acid receptor has high affinity for salmon calcitonin (dissociation constant Kd ≈ 6 nM) and is functionally coupled to increases in intracellular cyclic adenosine monophosphate (cAMP). The receptor shows no sequence similarity to other reported G protein-coupled receptors but is homologous to the parathyroid hormone-parathyroid hormone-related peptide (PTH-PTHrP) receptor, indicating that the receptors for these hormones, which regulate calcium homeostasis, represent a new family of G protein-coupled receptors.
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