Gamma interferon is able to enhance the oxidative metabolism of human neutrophils
G Berton, L Zeni, MA Cassatella, F Rossi - Biochemical and biophysical …, 1986 - Elsevier
G Berton, L Zeni, MA Cassatella, F Rossi
Biochemical and biophysical research communications, 1986•ElsevierThe oxidative metabolism of human neutrophils has been studied after incubation of the
cells with recombinant interferon-γ. Neutrophils incubated for 2–4 hours with 2–50 U/ml
recombinant interferon-γ undergo a higher respiratory burst measured both as O 2
consumption and O 2− production when stimulated with formyl-methionyl-leucyl-
phenylalanine, Concanavalin A or phorbol myristate acetate. The potentiating effect of
interferon-y requires more than one hour of incubation, is optimal at 20–50 U/ml and …
cells with recombinant interferon-γ. Neutrophils incubated for 2–4 hours with 2–50 U/ml
recombinant interferon-γ undergo a higher respiratory burst measured both as O 2
consumption and O 2− production when stimulated with formyl-methionyl-leucyl-
phenylalanine, Concanavalin A or phorbol myristate acetate. The potentiating effect of
interferon-y requires more than one hour of incubation, is optimal at 20–50 U/ml and …
Summary
The oxidative metabolism of human neutrophils has been studied after incubation of the cells with recombinant interferon-γ. Neutrophils incubated for 2–4 hours with 2–50 U/ml recombinant interferon-γ undergo a higher respiratory burst measured both as O2 consumption and O2− production when stimulated with formyl-methionyl-leucyl-phenylalanine, Concanavalin A or phorbol myristate acetate. The potentiating effect of interferon-y requires more than one hour of incubation, is optimal at 20–50 U/ml and depends on the presence of serum in the incubation medium. The interferon effect depends on new protein synthesis. Cycloheximide at doses which do not alter the respiratory response of normal cells completely prevents the potentiating effect of interferon.
Elsevier