[HTML][HTML] Thyrotropin receptor cleavage at site 1 does not involve a specific amino acid motif but instead depends on the presence of the unique, 50 amino acid …
K Tanaka, GD Chazenbalk, SM McLachlan… - Journal of Biological …, 1998 - ASBMB
Thyrotropin (TSH) receptor (TSHR) A and B subunits are formed by intramolecular cleavage
of the single chain receptor at two separate sites. The region involved in cleavage at Site 2
has been identified, but previous mutagenesis studies failed to identify Site 1. We now report
fortuitous observations on the effect of trypsin on the TSHR that localizes a small region
harboring Site 1. Thus, as detected by immunoblotting and by 125 I-TSH cross-linking to
TSHR expressed on the surface of intact CHO cells, trypsin clipped a small polypeptide …
of the single chain receptor at two separate sites. The region involved in cleavage at Site 2
has been identified, but previous mutagenesis studies failed to identify Site 1. We now report
fortuitous observations on the effect of trypsin on the TSHR that localizes a small region
harboring Site 1. Thus, as detected by immunoblotting and by 125 I-TSH cross-linking to
TSHR expressed on the surface of intact CHO cells, trypsin clipped a small polypeptide …