Real-time RT-PCR quantification of insulin-like growth factor (IGF)-1, IGF-1 receptor, IGF-2, IGF-2 receptor, insulin receptor, growth hormone receptor, IGF-binding …

MW Pfaffl, TM Georgieva, IP Georgiev… - Domestic animal …, 2002 - Elsevier
Reverse transcription (RT) followed by polymerase chain reaction (PCR) is the technique of
choice for analysing mRNA in extremely low abundance. Real-time RT-PCR using SYBR
Green I detection combines the ease and necessary exactness to be able to produce
reliable as well as rapid results. To obtain highly accurate and reliable results in a real-time
RT-PCR a highly defined calibration curve is needed. We designed and developed nine
different calibration curves, based on recombinant DNA plasmid standards and established …