An improved suicide vector for construction of chromosomal insertion mutations in bacteria
RJ Penfold, JM Pemberton - Gene, 1992 - Elsevier
RJ Penfold, JM Pemberton
Gene, 1992•ElsevierWe have constructed an R6K-based suicide vector (pJP5603) that requires a trans supply of
the pir-encoded π protein of plasmid R6K for replication. Therefore, efficient plasmid suicide
results upon transfer to bacteria not harbouring pir. The 3.1-kb vector encodes kanamycin
resistance and is mobilizable. When used in conjunction with a JM109 strain carrying pir, it
has nine unique restriction sites available for α-complementation cloning. Vector
functionality was demonstrated in Rhodobacter sphaeroides.
the pir-encoded π protein of plasmid R6K for replication. Therefore, efficient plasmid suicide
results upon transfer to bacteria not harbouring pir. The 3.1-kb vector encodes kanamycin
resistance and is mobilizable. When used in conjunction with a JM109 strain carrying pir, it
has nine unique restriction sites available for α-complementation cloning. Vector
functionality was demonstrated in Rhodobacter sphaeroides.
Abstract
We have constructed an R6K-based suicide vector (pJP5603) that requires a trans supply of the pir-encoded π protein of plasmid R6K for replication. Therefore, efficient plasmid suicide results upon transfer to bacteria not harbouring pir. The 3.1-kb vector encodes kanamycin resistance and is mobilizable. When used in conjunction with a JM109 strain carrying pir, it has nine unique restriction sites available for α-complementation cloning. Vector functionality was demonstrated in Rhodobacter sphaeroides.
Elsevier