Formation of short-lived protein aggregates directly from the coil in two-state folding

M Silow, YJ Tan, AR Fersht, M Oliveberg - Biochemistry, 1999 - ACS Publications
M Silow, YJ Tan, AR Fersht, M Oliveberg
Biochemistry, 1999ACS Publications
Recent results on the 102 residue protein U1A show that protein aggregation is not always
slow and irreversible but may take place transiently in refolding studies on a millisecond
time scale. In this study we observe a similar aggregation behavior with the classical two-
state protein CI2. Since both U1A and CI2 appear to fold directly from the coil at low protein
concentrations, it is likely that the aggregates also form directly from the coil. This is in
contrast to the behavior of larger multistate proteins where aggregation occurs in connection …
Recent results on the 102 residue protein U1A show that protein aggregation is not always slow and irreversible but may take place transiently in refolding studies on a millisecond time scale. In this study we observe a similar aggregation behavior with the classical two-state protein CI2. Since both U1A and CI2 appear to fold directly from the coil at low protein concentrations, it is likely that the aggregates also form directly from the coil. This is in contrast to the behavior of larger multistate proteins where aggregation occurs in connection to “sticky” intermediates.
ACS Publications