Experimental metastasis is suppressed in MMP-9-deficient mice
T Itoh, M Tanioka, H Matsuda, H Nishimoto… - Clinical & experimental …, 1999 - Springer
T Itoh, M Tanioka, H Matsuda, H Nishimoto, T Yoshioka, R Suzuki, M Uehira
Clinical & experimental metastasis, 1999•SpringerMatrix metalloproteinases (MMPs) are thought to play a key role in tumor invasion and
metastasis. The role of MMP-9 (gelatinase B) in tumor metastasis was examined in MMP-9-
deficient mice produced by gene targeting using embryonic stem cells. MMP-9-deficient
mice develop normally and are fertile. In these mice, the number of metastatic colonies of
B16-BL6 melanoma cells or Lewis lung carcinoma cells that were implanted intravenously
fell by 45% for B16-BL6 melanoma and 59% for Lewis lung carcinoma (p= 0.03 and p …
metastasis. The role of MMP-9 (gelatinase B) in tumor metastasis was examined in MMP-9-
deficient mice produced by gene targeting using embryonic stem cells. MMP-9-deficient
mice develop normally and are fertile. In these mice, the number of metastatic colonies of
B16-BL6 melanoma cells or Lewis lung carcinoma cells that were implanted intravenously
fell by 45% for B16-BL6 melanoma and 59% for Lewis lung carcinoma (p= 0.03 and p …
Abstract
Matrix metalloproteinases (MMPs) are thought to play a key role in tumor invasion and metastasis. The role of MMP-9 (gelatinase B) in tumor metastasis was examined in MMP-9-deficient mice produced by gene targeting using embryonic stem cells. MMP-9-deficient mice develop normally and are fertile. In these mice, the number of metastatic colonies of B16-BL6 melanoma cells or Lewis lung carcinoma cells that were implanted intravenously fell by 45% for B16-BL6 melanoma and 59% for Lewis lung carcinoma (p=0.03 and p=0.0043, respectively). Gelatin zymography showed that both tumor cell lines did not secrete MMP-9 by themselves but the host cells surrounding the tumor cells secrete MMP-9 in vivo. These results indicated that host-derived MMP-9 plays an important role in the process of tumor metastasis.
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