[5] IVET and RIVET: Use of gene fusions to identify bacterial virulence factors specifically induced in host tissues
JM Slauch, A Camilli - Methods in enzymology, 2000 - Elsevier
IVET was designed to identify those bacterial genes that are induced when a pathogen
infects its host. A subset of these induced genes encode virulence factors, products
specifically required for the infection process. The paradigm IVET system is based on
complementation of an attenuating auxotrophic mutation by gene fusion and is designed to
be of use in a wide variety of pathogenic organisms. In S. typhimurium, we have used this
system successfully to identify a number of genes that are induced in a BALB/c mouse and …
infects its host. A subset of these induced genes encode virulence factors, products
specifically required for the infection process. The paradigm IVET system is based on
complementation of an attenuating auxotrophic mutation by gene fusion and is designed to
be of use in a wide variety of pathogenic organisms. In S. typhimurium, we have used this
system successfully to identify a number of genes that are induced in a BALB/c mouse and …