[HTML][HTML] Role of Niemann-Pick type C1 protein in intracellular trafficking of low density lipoprotein-derived cholesterol
Niemann-Pick type C (NPC) is a disease that affects intracellular cholesterol-trafficking
pathways. By cloning the hamster ortholog of NPC1, we identified the molecular lesions in
two independently isolated Chinese hamster ovary cell mutants, CT60 and CT43. Both
mutants lead to premature translational terminations of the NPC1 protein. Transfecting
hamster NPC1cDNA complemented the defects of the mutants. Investigation of the CT
mutants, their parental cells, and an NPC1-stable transfectant allow us to present evidence …
pathways. By cloning the hamster ortholog of NPC1, we identified the molecular lesions in
two independently isolated Chinese hamster ovary cell mutants, CT60 and CT43. Both
mutants lead to premature translational terminations of the NPC1 protein. Transfecting
hamster NPC1cDNA complemented the defects of the mutants. Investigation of the CT
mutants, their parental cells, and an NPC1-stable transfectant allow us to present evidence …