[HTML][HTML] Vesicular and non-vesicular sterol transport in living cells: the endocytic recycling compartment is a major sterol storage organelle
M Hao, SX Lin, OJ Karylowski, D Wüstner… - Journal of Biological …, 2002 - ASBMB
We examined the intracellular transport of sterol in living cells using a naturally fluorescent
cholesterol analog, dehydroergosterol (DHE), which has been shown to mimic many of the
properties of cholesterol. By using DHE loaded on methyl-β-cyclodextrin, we followed this
cholesterol analog in pulse-chase studies. At steady state, DHE co-localizes extensively with
transferrin (Tf), a marker for the endocytic recycling compartment (ERC), and redistributes
with Tf in cells with altered ERC morphology. Expression of a dominant-negative mutation of …
cholesterol analog, dehydroergosterol (DHE), which has been shown to mimic many of the
properties of cholesterol. By using DHE loaded on methyl-β-cyclodextrin, we followed this
cholesterol analog in pulse-chase studies. At steady state, DHE co-localizes extensively with
transferrin (Tf), a marker for the endocytic recycling compartment (ERC), and redistributes
with Tf in cells with altered ERC morphology. Expression of a dominant-negative mutation of …