[HTML][HTML] Rapid nonvesicular transport of sterol between the plasma membrane domains of polarized hepatic cells
D Wustner, A Herrmann, M Hao, FR Maxfield - Journal of Biological …, 2002 - ASBMB
We studied the transport of the fluorescent cholesterol analog dehydroergosterol (DHE) in
polarized HepG2 human hepatoma cells. DHE delivered via methyl-β-cyclodextrin was
delivered to both the apical and basolateral membranes and became concentrated in the
apical membrane within 1 min. Intracellular DHE was targeted mainly to vesicles of the
subapical compartment or apical recycling compartment (SAC/ARC), where it colocalized
with fluorescent transferrin and fluorescent analogs of phosphatidylcholine and …
polarized HepG2 human hepatoma cells. DHE delivered via methyl-β-cyclodextrin was
delivered to both the apical and basolateral membranes and became concentrated in the
apical membrane within 1 min. Intracellular DHE was targeted mainly to vesicles of the
subapical compartment or apical recycling compartment (SAC/ARC), where it colocalized
with fluorescent transferrin and fluorescent analogs of phosphatidylcholine and …