Endotoxin inhibits heat induced Hsp70 in rats
JP Ofenstein, S Heidemann, A Juett… - Critical Care …, 1998 - journals.lww.com
Methods: 20 rats were anesthetized, cannulated and assigned to 5 treatment groups. Four
control rats received saline alone (group 1), four rats received LPS (E. Coli, 0127: B8,
2mg/kg) iv (group 2). four rats received heat shock (42 [degree sign] C) for 10 min (group 3),
four rats received LPS iv followed by heat shock (group 4), and four rats received heat shock
followed by LPS iv (group 5). Two hr after treatment, spleen was removed and a cell
suspension prepared by passing the spleen through a screen. Spleen cells were washed …
control rats received saline alone (group 1), four rats received LPS (E. Coli, 0127: B8,
2mg/kg) iv (group 2). four rats received heat shock (42 [degree sign] C) for 10 min (group 3),
four rats received LPS iv followed by heat shock (group 4), and four rats received heat shock
followed by LPS iv (group 5). Two hr after treatment, spleen was removed and a cell
suspension prepared by passing the spleen through a screen. Spleen cells were washed …